AI-guided stimuli discovery and generation to optimize facial emotion perception studies in autism

Understanding perceptual differences between autistic and neurotypical adults requires behavioral assays that are sensitive, reliable, and mechanistically informative. Facial emotion perception is a useful test case because group differences have been reported, but findings vary across studies. Here we show that this variability may reflect image-level sparsity: autistic-neurotypical differences in emotion judgments were concentrated in a small subset of diagnostic facial expressions rather than spread uniformly across stimuli. We trained population-specific artificial neural network models to predict image-level judgments for autistic and neurotypical participants, then used these models to select novel faces predicted to maximize group separation. In an independent cohort, model-selected images produced larger behavioral differences than matched random images. We then used the same models with a generative adversarial network to transform diagnostic images toward greater predicted group agreement. In phenotype-matched validation, synthesized images reduced behavioral separation relative to their matched originals. These results establish a model-guided framework for discovering and transforming stimuli that reveal population-specific perceptual differences. More broadly, they show how behavioral phenotyping can move beyond averaging across fixed stimulus sets toward optimized assays that identify the conditions under which neurodivergent perception diverges or converges.

Automated 3D Kinematic Monitoring for Circadian Activity and Anomaly Detection in Juvenile Fish

Precision aquaculture faces a "phenotyping bottleneck" in tracking high-resolution behavioral traits, as conventional methods cannot quantify instantaneous three-dimensional (3D) physical exertion. To address this, we present a high-throughput 3D behavioral phenotyping framework integrating deep learning object detection with binocular stereo vision for real-time monitoring of juvenile tilapia in high-density environments. The system automates non-contact body length estimation and reconstructs 3D swimming trajectories from absolute spatial coordinates. By eliminating 2D perspective distortions, this approach precisely quantifies 3D velocity and acceleration, marking the first estimation of true physical swimming speeds in free-roaming juveniles. Results show the framework successfully establishes circadian locomotor baselines, serving as an early warning system for physiological stress and providing an objective metric for fish vitality.