Clinical Feasibility of Low-Magnification Fluorescence Imaging for Breast Cancer Margin Detection Using Texture Analysis and Deep Learning

High-resolution images of unprocessed surgical breast tissue can be obtained using microscopy with ultraviolet surface excitation (MUSE). This technique is considered a promising method for checking surgical margins during breast cancer surgery. In this study, MUSE images at 4x and 10x magnifications were compared using patch-level classification methods. Texture analysis (TA) based on local binary patterns (LBP) and deep learning (DL) with a base Vision Transformer (ViT) model were used. Both methods achieved similar performance at both magnifications. Using DL method, both 4x and 10x magnifications achieved 96.30% sensitivity, 100% specificity and 98.18% accuracy. Using TA method, 4x achieved better specificity (100% vs 93.33%) and 10x yielded higher sensitivity (100% vs 93.33%), but both had the same accuracy (96.67%). No clear improvement in performance was observed with 10x magnification. These results show that 4x imaging achieves the same diagnostic accuracy as 10x imaging. At the same time, 4x offers a larger field of view and faster image capture. Therefore, lower magnification can be effectively used in MUSE systems for accurate and efficient intraoperative margin assessment.

A second-order theory of texture for depth from focus

We present a theory of textured appearance of optically rough surfaces based on wave optics, emphasizing the role of texture for passive depth from focus. Our theory shows that even surfaces that traditional computer vision would consider textureless can produce textured appearance, due to subjective speckle from surface microgeometry. We analyze the properties of this second-order texture, and show that we can enhance its contrast under natural ambient lighting by simply using a narrowband spectral filter. Doing so results in dramatic improvements in passive depth reconstruction of seemingly textureless scenes, as we demonstrate through extensive theory, simulations, and real-world experiments.

Physically Aware Radiomics Without Interpolation: Disentangling Voxel Geometry and Signal Modification in CT and MRI

Objective: Radiomic texture features are usually computed in voxel-index neighborhoods, implicitly assuming isotropic spatial relationships. In anisotropic images, this can confound voxel geometry with interpolation-induced signal changes. We developed a voxel-spacing-aware radiomic framework that incorporates physical geometry into texture computation without resampling. Approach: We modified PyRadiomics to account for voxel spacing while preserving the native image signal. Four configurations were compared: native non-resampled extraction (NR), isotropic resampling (RS), voxel-spacing-aware extraction (VS), and fake-isotropic preprocessing (FK), in which spacing metadata were overwritten without altering the image array. Experiments included 685 LIDC-IDRI pulmonary nodules and 209 I-SPY2 breast MRI cases, with 196 radiomic descriptors. Robustness was assessed using ICC, within-subject variability, Friedman testing, feature selection, machine learning, a multilayer perceptron, and external validation. Main results: VS showed near-native agreement with NR: median ICC(A,1) was 0.9976 in CT and 0.9984 in MRI. RS produced lower agreement and larger deviations, while FK showed intermediate behavior, confirming that spacing metadata alone can affect radiomic features. Gradient-derived and neighborhood-sensitive descriptors were most affected by preprocessing. VS preserved predictive performance comparable to NR in external CT validation, whereas MRI showed greater variability across preprocessing strategies and classifiers. Significance: Voxel-spacing-aware extraction separates geometric modeling from interpolation-induced signal modification while preserving the native image signal, offering a coherent alternative to isotropic resampling for radiomic analysis of anisotropic CT and MRI.

Revisiting One-Zero and Two-Zero Neutrino Mass Textures in Light of Recent Oscillation and Cosmological Data

We revisit one-zero and two-zero textures of the neutrino mass matrix under current experimental and cosmological constraints. We identify the phenomenologically viable texture structures using the latest results on neutrino oscillation parameters, the cosmological bound on the sum of neutrino masses, the kinematic bound on the effective electron-neutrino mass, and limits from neutrinoless double-beta decay. For two-zero textures, several structures are still allowed if only the CMB bound on the neutrino mass sum is imposed. Among them, the $B$-series textures show a characteristic prediction for the Dirac CP phase, with $δ_{\rm CP}$ lying around $π/2$ and $3π/2$, and are within the reach of future neutrinoless double-beta decay searches. When the stronger CMB+BAO constraint is included, however, only the $A$-series textures remain viable. Therefore, we also analyze one-zero textures by using machine learning techniques, particularly flow matching. It turns out that some of the texture structures are already excluded by current data, while the allowed ones give distinct predictions for $\sum_i m_i$, $m_{ν_e}^{\rm eff}$, $\langle m_{ee}\rangle$, and $δ_{\rm CP}$. We further discuss how the one-zero texture structures can arise from non-invertible selection rules.